Peptidylglycine monooxygenase

Peptidylglycine alpha amidating monooxygenase assay

Hearts were dissected into ventricles and atria, and were quick-frozen. The remainder was quick-frozen in liquid nitrogen.

Each rat sampled came from a

Pregnant mice were also placed on treatment after mating. Each rat sampled came from a separate litter. Sample collection After light ether anesthesia, and following decapitiation, trunk blood was drawn from rats and mice into heparinized microhematocrit tubes. Antisera was a gift from G.

One of these essential nutrients is the transition metal, copper. The transfected proteins were examined using enzyme assays, subcellular fractionation, Western blotting, and immunocytochemistry.

Pregnant mice were also

The antisera were a gift from B. In most cases, it appears as though the steady-state concentration of copper-binding proteins is lower following copper deficiency.

Sample collection After light ether anesthesiaMembranes were stained with Ponceau S

Plasma was obtained by centrifugation. Membranes were stained with Ponceau S Sigma Chemical to document equal protein loading.

Plasma was obtained by centrifugation