Peptidylglycine alpha amidating monooxygenase assay
Hearts were dissected into ventricles and atria, and were quick-frozen. The remainder was quick-frozen in liquid nitrogen.
Pregnant mice were also placed on treatment after mating. Each rat sampled came from a separate litter. Sample collection After light ether anesthesia, and following decapitiation, trunk blood was drawn from rats and mice into heparinized microhematocrit tubes. Antisera was a gift from G.
One of these essential nutrients is the transition metal, copper. The transfected proteins were examined using enzyme assays, subcellular fractionation, Western blotting, and immunocytochemistry.
The antisera were a gift from B. In most cases, it appears as though the steady-state concentration of copper-binding proteins is lower following copper deficiency.
Plasma was obtained by centrifugation. Membranes were stained with Ponceau S Sigma Chemical to document equal protein loading.
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